PXD043135 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | STPP-UP: A alternative method for drug target identification using protein thermal stability |
Description | Thermal proteome profiling (TPP) has significantly advanced the field of drug discovery by facilitating proteome-wide identification of drug targets and off-targets. However, TPP has not been widely applied for high-throughput drug screenings, since the method is labor intensive and requires a lot of measurement time on a mass spectrometer. Here, we present Single-tube TPP with Uniform Progression (STPP-UP), which significantly reduces both the amount of required input material and measurement time, while retaining the ability to identify drug targets for compounds of interest. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:38.553.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dick Zijlmans |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-20 03:55:11 | ID requested | |
1 | 2023-10-24 05:43:58 | announced | |
⏵ 2 | 2023-11-14 08:25:38 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: protein stability, drug discovery, thermal proteomics,STPP-UP |
Contact List
Michiel Vermeulen |
contact affiliation | Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands. Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands. |
contact email | michiel.vermeulen@ru.nl |
lab head | |
Dick Zijlmans |
contact affiliation | Radboud Universiteit Nijmegen |
contact email | d.zijlmans@science.ru.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043135
- Label: PRIDE project
- Name: STPP-UP: A alternative method for drug target identification using protein thermal stability