PXD043112
PXD043112 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation |
| Description | Glycolysis is a fundamental cellular process, yet its regulatory mechanisms remain incompletely understood. Here, we show that a subset of glucose transporter 1 (GLUT1/SLC2A1) co-endocytoses with platelet-derived growth factor (PDGF) receptor (PDGFR) upon PDGF-stimulation. LC-MS was used to characterize the isolates from cells stimulated with PDGF-BB-conjugated nanoparticles (endocytic vesicle fraction) or PDGF-BB plus unconjugated nanoparticles (control). Taken together, multiple glycolytic enzymes localize to these endocytosed PDGFR/GLUT1-containing vesicles adjacent to mitochondria. Contrary to current models, which emphasize the importance of glucose transporters on the cell surface, we find that PDGF-stimulated glucose uptake depends on receptor/transporter endocytosis. Our results suggest that growth factors generate glucose-loaded endocytic vesicles that deliver glucose to the glycolytic machinery in proximity to mitochondria, and argue for a new layer of regulation for glycolytic control governed by cellular membrane dynamics. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-09-19 |
| AnnouncementXML | Submission_2023-09-19_17:24:33.405.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Beatrix Ueberheide |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-19 12:49:13 | ID requested | |
| ⏵ 1 | 2023-09-19 17:24:33 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Glycolysis, glucose transporter 1, endocytic vesicles |
Contact List
| Beatrix Ueberheide | |
|---|---|
| contact affiliation | NYU School of Medicine |
| contact email | beatrix.ueberheide@nyulangone.org |
| lab head | |
| Beatrix Ueberheide | |
| contact affiliation | NYU School of Medicine |
| contact email | beatrixueberheide@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092208/ |
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