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PXD043076

PXD043076 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells
DescriptionThe devastating blast fungus Magnaporthe oryzae elaborates invasive hyphae (IH) in living rice cells during early infection, separated from host cytoplasm by plant-derived interfacial membranes, but the metabolic strategies underpinning this fundamental intracellular biotrophic growth phase are poorly understood. Eukaryotic cell growth depends on activated target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Here, using live-cell imaging coupled with multiomic approaches, we show how the M. oryzae serine/threonine protein kinase Rim15 coordinates cycles of autophagy and glutaminolysis in IH – the latter through phosphorylation of NAD-dependent glutamate dehydrogenase – to reactivate TOR and promote biotrophic growth. Deleting RIM15 attenuated IH growth and triggered plant immunity; these defects were fully remediated by exogenous α-ketoglutarate treatment, but glucose treatment only suppressed host defenses. Our results together suggest that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression.
HostingRepositoryPRIDE
AnnounceDate2023-07-10
AnnouncementXMLSubmission_2023-07-10_12:31:04.341.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGangLi
SpeciesList scientific name: Magnaporthe sp. MG08; NCBI TaxID: 1758543;
ModificationListphosphorylated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-18 08:48:25ID requested
12023-07-10 12:31:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: glutaminolysis, autophagy, α-ketoglutarate, antioxidation, TOR signaling, biotrophy, Rim15,Rice blast disease, Magnaporthe oryzae, biotrophic interface, plant defense suppression
Contact List
Richard A.Wilson
contact affiliationDepartment of Plant Pathology, University of Nebraska, Lincoln,NE
contact emailrwilson10@unl.edu
lab head
GangLi
contact affiliationUniversity of Nebraska-Lincoln
contact emaillig.genetics@gmail.com
dataset submitter
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Dataset FTP location
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