PXD043076 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells |
Description | The devastating blast fungus Magnaporthe oryzae elaborates invasive hyphae (IH) in living rice cells during early infection, separated from host cytoplasm by plant-derived interfacial membranes, but the metabolic strategies underpinning this fundamental intracellular biotrophic growth phase are poorly understood. Eukaryotic cell growth depends on activated target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Here, using live-cell imaging coupled with multiomic approaches, we show how the M. oryzae serine/threonine protein kinase Rim15 coordinates cycles of autophagy and glutaminolysis in IH – the latter through phosphorylation of NAD-dependent glutamate dehydrogenase – to reactivate TOR and promote biotrophic growth. Deleting RIM15 attenuated IH growth and triggered plant immunity; these defects were fully remediated by exogenous α-ketoglutarate treatment, but glucose treatment only suppressed host defenses. Our results together suggest that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-10 |
AnnouncementXML | Submission_2023-07-10_12:31:04.341.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | GangLi |
SpeciesList | scientific name: Magnaporthe sp. MG08; NCBI TaxID: 1758543; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-18 08:48:25 | ID requested | |
⏵ 1 | 2023-07-10 12:31:04 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: glutaminolysis, autophagy, α-ketoglutarate, antioxidation, TOR signaling, biotrophy, Rim15,Rice blast disease, Magnaporthe oryzae, biotrophic interface, plant defense suppression |
Contact List
Richard A.Wilson |
contact affiliation | Department of Plant Pathology, University of Nebraska, Lincoln,NE |
contact email | rwilson10@unl.edu |
lab head | |
GangLi |
contact affiliation | University of Nebraska-Lincoln |
contact email | lig.genetics@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043076
- Label: PRIDE project
- Name: A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells