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PXD043038

PXD043038 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePIAS3/TGF-β/TXNIP axis promotes ferroptosis in HCC
DescriptionFerroptosis has been suggested to have a potential role in cancer therapy as an iron-dependent programmed cell death distinguished from other forms. Hepatocellular carcinoma (HCC) remains a great threat with high lethality and limited therapies. Induction of ferroptosis has emerged as a novel and promising therapeutic strategy for HCC. Here, we identify PIAS3 as a driver of ferroptosis in HCC through TMT-based quantitative proteomics and ferroptosis-related functional assays. Mechanistically, TXNIP is confirmed as a downstream of PIAS3 in promoting ferroptotic cell death based on RNA-seq analysis. The knockdown of TXNIP degrades ferroptotic susceptibility caused by PIAS3-overexpression, whereas the transfection-forced re-expression of TXNIP restores sensitivity to ferroptosis in PIAS3-downregulated cells. In the meantime, PIAS3 interacts with SMAD2/3 to activate TGF-β signaling, which leads to increased TXNIP expression. Our study uncovers the critical role of PIAS3 in ferroptosis and a novel actionable axis-PIAS3/TGF-β/TXNIP that could govern ferroptotic sensitivity, paving the path for targeting ferroptosis as an efficient approach in HCC therapies.
HostingRepositoryiProX
AnnounceDate2023-06-15
AnnouncementXMLSubmission_2023-06-15_18:50:12.903.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJingde Chen
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-15 18:49:53ID requested
12023-06-15 18:50:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PIAS3, Ferroptosis, HCC, TGF-β signaling, TXNIP
Contact List
Jingde Chen
contact affiliationShanghai East Hospital, Tongji University School of Medicine
contact email1600092@tongji.edu.cn
lab head
Jingde Chen
contact affiliationShanghai East Hospital, Tongji University School of Medicine
contact email1600092@tongji.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI