PXD042947
PXD042947 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome |
Description | Membrane-bound particles in plasma are composed of exosomes, microvesicles, and apoptotic bodies and represent ~1-2% of the total protein composition. Proteomic interrogation of this subset of plasma proteins augments the representation of tissue-specific proteins, representing a “liquid biopsy,” while enabling the detection of proteins that would otherwise be beyond the dynamic range of liquid chromatography-tandem mass spectrometry in unfractionated plasma. We have developed a one-step enrichment strategy (Mag-Net) using hyper-porous strong-anion exchange magnetic microparticles to sieve membrane-bound particles from plasma. The Mag-Net method is robust, reproducible, inexpensive, and requires <100 μL plasma input. Coupled to a quantitative data-independent mass spectrometry analytical strategy, we demonstrate that we can routinely collect results for >37,000 peptides from >4,000 plasma proteins with high precision. We demonstrate excellent quantitative accuracy and analytical reproducibility of the protocol. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2023-06-13 |
AnnouncementXML | Submission_2023-06-13_15:35:37.964.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Michael MacCoss |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-06-13 09:40:48 | ID requested | |
⏵ 1 | 2023-06-13 15:35:38 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: extracellular vesicles, DIA mass spectrometry, plasma proteomics, quantitative proteomics, neurodegenerative disease |
Contact List
Michael MacCoss | |
---|---|
contact affiliation | University of Washington |
contact email | maccoss@uw.edu |
lab head | |
Michael MacCoss | |
contact affiliation | University of Washington |
contact email | maccoss@uw.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |