PXD042905 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The assembly of the Mitochondrial Complex I Assembly complex uncovers a redox pathway coordination |
Description | The Mitochondrial Complex I Assembly (MCIA) complex is essential for the biogenesis of respiratory Complex I (CI), the first enzyme in the respiratory chain, which has been linked to Alzheimer’s disease (AD) pathogenesis. However, how MCIA facilitates CI assembly, and how it is linked with AD pathogenesis, is poorly understood. Here we report the structural basis of the complex formation between the MCIA subunits ECSIT and ACAD9. ECSIT binding induces a major conformational change in the FAD-binding loop of ACAD9, releasing the FAD cofactor and converting ACAD9 from a fatty acid β-oxidation (FAO) enzyme to a CI assembly factor. We provide evidence that ECSIT phosphorylation downregulates its association with ACAD9 and is reduced in neuronal cells upon exposure to amyloid-β (Aβ) oligomers. These findings advance our understanding of the MCIA complex assembly and suggest a possible role for ECSIT in the reprogramming of bioenergetic pathways linked to Aβ toxicity, a hallmark of AD. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:17:32.844.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jennifer Schwarz |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-12 03:58:35 | ID requested | |
1 | 2023-12-13 08:19:58 | announced | |
⏵ 2 | 2024-10-22 06:17:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Keywords: Oxidative phosphorylation (OXPHOS), Mitochondrial Complex I Assembly Complex (MCIA), Fatty Acid Oxidation (FAO), Amyloid-β, ECSIT phosphorylation, Cryo-EM |
Contact List
Montserrat Soler-Lopez |
contact affiliation | Structural Biology Group, European Synchrotron Radiation Facility (ESRF), Grenoble, France |
contact email | montserrat.soler-lopez@esrf.fr |
lab head | |
Jennifer Schwarz |
contact affiliation | European Molecular Biology Laboratory, Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany |
contact email | jennifer.schwarz@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042905
- Label: PRIDE project
- Name: The assembly of the Mitochondrial Complex I Assembly complex uncovers a redox pathway coordination