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PXD042871

PXD042871 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleValidity of the cell-extracted proteome as a substrate pool for exploring phosphorylation motifs of kinases
DescriptionThree representative protein kinases with different substrate preference including ERK1 (pro-directed), CK2 (acidophilic), and PKA (basophilic) and the substrate pool proteome from three different cell lines including MCF7 (human mammary carcinoma), HeLa (human cervical carcinoma), Jurkat (human acute T-cell leukemia) were used to evaluate phosphorylation sequence motifs. Specifically, recombinant kinases were added to the cell-extracted proteome, phosphorylated in vitro, trypsin digested, and the phosphopeptides were enriched for nanoLC/MS/MS analysis to identify their phosphorylation sites on a large scale. By analyzing the obtained phosphorylation sites and their surrounding sequences, phosphorylation motifs were extracted for each kinase-substrate proteome pair. The results showed that each kinase exhibited identical phosphorylation motifs, independent of the substrate proteome. Furthermore, the motifs were also consistent with those from completely randomized peptide libraries. These results indicate that cell-extracted proteomes can provide kinase phosphorylation motifs with sufficient accuracy, even though their sequences are not completely random, supporting the robustness of the phosphorylation motifs from the phosphoproteome analysis of cell extracts as a substrate pool for the kinase of interest.
HostingRepositoryjPOST
AnnounceDate2024-06-11
AnnouncementXMLSubmission_2024-06-10_08:00:20.321.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTomoya Niinae
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-11 00:59:10ID requested
12024-06-10 08:00:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Kinase, Phosphoproteome
Contact List
Yasushi Ishihama
lab head
Tomoya Niinae
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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