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PXD042770

PXD042770 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS
DescriptionData-Independent Acquisition (DIA) is a mass spectrometry-based method to reliably identify and reproducibly quantify large fractions of a target proteome. The peptide-centric data analysis strategy employed in DIA requires a priori generated spectral assay libraries. Such assay libraries allow to extract quantitative data in a targeted approach and have been generated for human, mouse, zebrafish, E. coli and few other organisms. However, a spectral assay library for the extreme halophilic archaeon Halobacterium salinarum NRC-1, a model organism that contributed to several notable discoveries, is not publicly available yet. Here, we report a comprehensive spectral assay library to measure 2,563 of 2,646 annotated H. salinarum NRC-1 proteins. We demonstrate the utility of this library by measuring global protein abundances over time under standard growth conditions. The H. salinarum NRC-1 library includes 21,074 distinct peptides representing 97% of the predicted proteome and provides a new, valuable resource to confidently measure and quantify any protein of this archaeon.
HostingRepositoryPRIDE
AnnounceDate2023-09-21
AnnouncementXMLSubmission_2023-09-21_00:37:09.915.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUlrikeKusebauch
SpeciesList scientific name: Halobacterium salinarum; NCBI TaxID: 2242;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-06 11:42:15ID requested
12023-09-21 00:37:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DIA, Data-independent acquisition, SWATH,Halobacterium salinarum NRC-1, spectral assay library, DDA, data-dependent acquisition
Contact List
RobertL.
contact affiliationInstitute for Systems Biology, Seattle, WA, USA
contact emailrmoritz@systemsbiology.org
lab head
UlrikeKusebauch
contact affiliationInstitute for Systems Biology, Seattle, WA, USA
contact emailukusebauch@systemsbiology.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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