PXD042503

PXD042503 is an original dataset announced via ProteomeXchange.

Title	Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria.
Description	Severe malaria (SM) is a life-threatening condition caused by Plasmodium falciparum and the most severe presentation is cerebral malaria (CM) mostly affecting children under five years old. The comprehensive understanding of the complex mechanisms driving the pathophysiology of CM, which encompasses both host and parasite factors, remains insufficiently elucidated. This study analyzed clinical isolates from Beninese children using liquid chromatography-mass spectrometry to identify differentially expressed proteins in SM compared to uncomplicated malaria (UM) patients. We showed a down-regulation of proteins involved in ubiquitination and proteasomal protein degradation in infected erythrocytes from CM patients. In plasma we observed that proteasome 20S components were more abundant in SM patients, which could potentially be useful as a severity biomarker. Furthermore, transferrin receptor protein 1 was specifically upregulated in CM infected erythrocyte isolates which raises the hypothesis that parasites causing CM could preferably infect reticulocytes or erythroid precursors. Consistently with this hypothesis we also found that parasite proteins implicated in distinct biosynthesis pathways were upregulated in CM. Moreover, functional analysis showed deregulated iron metabolism and ferroptosis pathways associated with CM presentation. Further investigations are required to confirm these findings and determine their potential for clinical application.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:17.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Salnot
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-25 10:54:40	ID requested
1	2024-02-01 05:48:46	announced
⏵ 2	2024-10-22 06:28:17	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Infected erythrocytes,Severe malaria, Plasma, Infected erythrocytes Severe malaria, Plasmodium falciparum, LC-MS/MS

Gwladys I.
contact affiliation	Université Paris Cité, IRD, UMR261, MERIT, F-75006 Paris,France.
contact email	gwladys.bertin@ird.fr
lab head
Virginie Salnot
contact affiliation	3p5-proteom'ic plateform
contact email	virginie.salnot@parisdescartes.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042503
     1. Label: PRIDE project
     2. Name: Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria.