PXD042487

PXD042487 is an original dataset announced via ProteomeXchange.

Title	The CprRS two-component system regulates virulence in Pseudomonas aeruginosa
Description	The ability of Pseudomonas aeruginosa to detect host immune responses and coordinate the production of virulence factors is crucial for its high pathogenicity. However, the regulatory mechanisms underlying this process remain largely unknown. In this study, we extensively characterize the conserved two-component system CprRS in P. aeruginosa and uncover the role of the sensor histidine kinase CprS as a receptor for the human host defense peptide LL-37, thereby modulating bacterial virulence. In the presence of LL-37, CprS functions as a phosphatase, targeting the intracellular activator CprR for phosphorylation at position D53, ultimately leading to CprR activation. By employing quantitative proteomics and transcription analysis, we further elucidate that CprR induction under LL-37 treatment promotes the expression of type III secretion system effectors. This, in turn, impedes the expression of proinflammatory cytokines and significantly increases bacterial cytotoxicity towards macrophages. Notably, the mutation of either cprS or cprR markedly diminishes bacterial survival in both macrophage and insect infection models. Collectively, our study uncovers a novel regulatory mechanism of the two-component system in P. aeruginosa, which enables the bacterium to sense and respond to human innate immune system responses while ensuring a timely balance of virulence genes.
HostingRepository	iProX
AnnounceDate	2023-05-25
AnnouncementXML	Submission_2023-05-24_19:53:39.801.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siping Zhang
SpeciesList	scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-24 19:49:19	ID requested
1	2023-05-24 19:49:26	announced
⏵ 2	2023-05-24 19:53:40	announced	2023-05-25: Update information.

Dataset with its publication pending

submitter keyword: P. aeruginosa, two-component system, LL-37, type III secretion system

Heyong Xing
contact affiliation	Lanzhou University
contact email	heyx@lzu.edu.cn
lab head
Siping Zhang
contact affiliation	Lanzhou University
contact email	zhangsp2015@lzu.edu.cn
dataset submitter

iProX dataset URI