PXD042439

PXD042439 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Robust and scalable fluorosequencing technology
Description	Title: Robust and scalable fluorosequencing technology Corresponding Author: Jagannath Swaminathan HLA peptides from mono-allelic B-cell line using tandem mass-spectrometry We purchased the monoallelic B-cell line (B721.221), with HLA allele A*2603, directly from the International Histocompatibility Working group (Seattle, Washington). We cultured the cells and isolated HLA peptides from approximately 300 million cells as previously described (Abelin et al. 2017). We identified the isolated HLA peptides using an LC-coupled tandem mass-spectrometer (ThermoFisher, Orbitrap Fusion Lumos) and a reference dataset of a human proteome (Swissprot), applying settings from the literature for analyzing HLA peptides (Bassani-Sternberg et al. 2015) and ProteomeDiscoverer 2.3 software (Thermofisher).
HostingRepository	MassIVE
AnnounceDate	2023-08-08
AnnouncementXML	Submission_2023-08-08_09:28:51.400.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jagannath Swaminathan
SpeciesList	scientific name: Human;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-23 10:19:34	ID requested
⏵ 1	2023-08-08 09:28:51	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: HLA, Human leukocyte antigen, single molecule protein sequencing

submitter keyword: HLA, Human leukocyte antigen, single molecule protein sequencing

Contact List

Jagannath Swaminathan
contact affiliation	Erisyon Inc
contact email	jagannath@erisyon.com
lab head
Jagannath Swaminathan
contact affiliation	Erisyon Inc
contact email	jagannath@erisyon.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092015/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092015/