PXD042427

PXD042427 is an original dataset announced via ProteomeXchange.

Title	Obatoclax rescues FUS-ALS phenotypes in iPSC-derived neurons by inducing autophagy
Description	Disruption of protein homeostasis plays an essential role in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). One strategy for restoring protein homeostasis and protecting neurons is to de-repress autophagy by disrupting the inhibitory BECN1/BCL2 complex, which has been shown to improve healthspan and lifespan in mice. We screened small molecule BH3 mimetics using an induced pluripotent stem cell (iPSC)-based model of ALS with a FUS mutation and identified obatoclax rescued neurons at by reducing cytoplasmic FUS levels, restoring protein homeostasis, and reducing degeneration.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:31:27.622.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Gentzel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-23 05:10:47	ID requested
1	2023-10-16 03:55:43	announced
⏵ 2	2023-11-14 08:31:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: DIA, Autophagy, iPSC-derived neurons, ALS, BH3 mimetic,Label-free Quantitative Mass Spectrometry, FUS

Prof. Dr. Jared Sterneckert
contact affiliation	Center for Regenerative Therapies TU Dresden (CRTD), Technische Universität Dresden, Fetscherstraße 105, 01307, Dresden, Germany
contact email	jared.sterneckert@tu-dresden.de
lab head
Marc Gentzel
contact affiliation	Molecular Analysis - Mass Spectrometry, Center for Molecular and Cellular Bioengineering (CMCB), TU Dresden
contact email	marc.gentzel@tu-dresden.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042427

PRIDE project URI

• PRIDE
  1. PXD042427
     1. Label: PRIDE project
     2. Name: Obatoclax rescues FUS-ALS phenotypes in iPSC-derived neurons by inducing autophagy