PXD042334

PXD042334 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SoDA-PAL Protein Profiling Proteomics of Sorgoleone Catabolizing Microbial Isolate Acinetobacter pittii SO1
Description	Photoaffinity probe-based protein labeling of microbial isolate Acinetobacter pittii SO1 (NCBITAXON:48296), isolated from the sorghum rhizosphere that can utilize sorgoleone as a sole carbon source in order to identify proteins that bind sorgoleone. Photoaffinity labeling of A. pittii lysates using the SoDA-PAL probe approach may be extended in the future to proteomic profiling of complex rhizosphere microbiomes to discover genes that can be leveraged to promote beneficial plant-microbe interactions (grown on both acetate and purified sorgoleone). Eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization for subsequent quantitative proteomic analysis.
HostingRepository	MassIVE
AnnounceDate	2024-09-04
AnnouncementXML	Submission_2024-09-04_15:47:25.967.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Acinetobacter pittii; NCBI TaxID: 48296;
ModificationList	Oxidation
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-18 10:23:51	ID requested
⏵ 1	2024-09-04 15:47:26	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: sorghum rhizosphere, microbial proteomics, LC-MS, peptide quantitation, PerCon SFA, SoDA-PAL probe proteomics, sorgoleone catabolism

submitter keyword: sorghum rhizosphere, microbial proteomics, LC-MS, peptide quantitation, PerCon SFA, SoDA-PAL probe proteomics, sorgoleone catabolism

Contact List

Robert G. Egbert
contact affiliation	Pacific Northwest National Laboratory
contact email	robert.egbert@pnnl.gov
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091986/