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PXD042231

PXD042231 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe HLA-II immunopeptidome of SARS-CoV-2
DescriptionWeingarten-Gabbay S, Chen D, Sarkizova S, Taylor HB, Gentili M, Pearlman LR, Bauer MR, Rice CM, Clauser KR, Hacohen N, Carr SA, Abelin JG, Saeed M, Sabeti PC. 2023. Targeted synthetic vaccines have the potential to transform our response to viral outbreaks; yet the design of these vaccines requires a comprehensive knowledge of viral immunogens, including T-cell epitopes. Having previously mapped the SARS-CoV-2 HLA-I landscape, here we report viral peptides that are naturally processed and loaded onto HLA-II complexes in infected cells. We identified over 500 unique viral peptides from canonical proteins, as well as overlapping internal open reading frames (ORFs), revealing, for the first time, the contribution of internal ORFs to the HLA-II peptide repertoire. Most HLA-II peptides co-localized with the known CD4+ T cell epitopes in COVID-19 patients, including a finding that two reported immunodominant regions in the SARS-CoV-2 membrane protein were formed at the level of HLA-II presentation. We observed that the HLA-I and HLA-II pathways target distinct viral proteins, with the structural proteins accounting for the majority of the HLA-II peptidome and non-structural and non-canonical proteins accounting for the majority of the HLA-I peptidome. These findings suggest that the future vaccines should incorporate multiple viral elements harboring CD4+ and CD8+ T cell epitopes to maximize their effectiveness.
HostingRepositoryMassIVE
AnnounceDate2024-01-11
AnnouncementXMLSubmission_2024-01-11_09:22:03.833.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarl Clauser
SpeciesList scientific name: SARS-CoV-2; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListunknown modification; unknown modification; unknown modification
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-15 06:02:25ID requested
12024-01-11 09:22:04announced
Publication List
no publication
Keyword List
submitter keyword: immunopeptidome, SARS-CoV-2
Contact List
Steven A. Carr
contact affiliationBroad Institute of MIT and Harvard
contact emailscarr@broadinstitute.org
lab head
Karl Clauser
contact affiliationBroad Institute of MIT and Harvard
contact emailclauser@broadinstitute.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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