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PXD041978

PXD041978 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach
DescriptionThe gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicated that HP1021 modulates H. pylori's response to oxidative stress. HP1021 controlled the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins were differently expressed in the HP1021 deletion mutant. HP1021 regulated typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. We identified HP1021 as the first molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes was relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controlled glucose consumption by directly regulating the gluP transporter and had an important impact on maintaining the energetic balance in the cell.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:03:27.886.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristina Ludwig
SpeciesList scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-04 05:59:55ID requested
12023-09-20 06:34:19announced
22024-10-22 06:03:28announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: glucose consumption,Helicobacter pylori, HP1021, oxidative stress
Contact List
Christina Ludwig
contact affiliationBavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University of Munich (TUM), 85354 Freising, Germany
contact emailtina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliationTU Munich
contact emailtina.ludwig@tum.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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