PXD041978 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach |
Description | The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicated that HP1021 modulates H. pylori's response to oxidative stress. HP1021 controlled the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins were differently expressed in the HP1021 deletion mutant. HP1021 regulated typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. We identified HP1021 as the first molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes was relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controlled glucose consumption by directly regulating the gluP transporter and had an important impact on maintaining the energetic balance in the cell. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:03:27.886.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christina Ludwig |
SpeciesList | scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-04 05:59:55 | ID requested | |
1 | 2023-09-20 06:34:19 | announced | |
⏵ 2 | 2024-10-22 06:03:28 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: glucose consumption,Helicobacter pylori, HP1021, oxidative stress |
Contact List
Christina Ludwig |
contact affiliation | Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University of Munich (TUM), 85354 Freising, Germany |
contact email | tina.ludwig@tum.de |
lab head | |
Christina Ludwig |
contact affiliation | TU Munich |
contact email | tina.ludwig@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041978
- Label: PRIDE project
- Name: Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach