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PXD041951

PXD041951 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFlavodoxin Landscape in Clostridioides difficile
DescriptionC. difficile infections have become a major challenge in medical facilities today. The bacterium is capable of spore formation, which even allows survival of antibiotic treatment. Therefore, research on the physiology of C. difficile is important for the development of alternative treatment strategies. In this study, we investigated eight putative flavodoxins of C. difficile 630. Flavodoxins are small electron transfer proteins of specifically low potential. The unusually high number of flavodoxins in C. difficile compared to other bacteria suggests that they are expressed under different conditions. Therefore, we investigated expression along bacterial growth and found high transcription levels during the exponential growth phase, especially for floX. Since flavodoxins in other bacteria are capable of replacing ferredoxins with their [2Fe-2S] clusters under iron deficiency conditions, we also examined flavodoxin expression in C. difficile under low iron and no iron levels.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:28:23.286.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniela Zuehlke
SpeciesList scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-03 09:15:44ID requested
12024-02-02 00:53:57announced
22024-10-22 06:28:23announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: expression profiling,mass spectrometry, parallel reaction monitoring
Contact List
Susanne Sievers
contact affiliationUniversity of Greifswald, Institute of Microbiology
contact emailsusanne.sievers@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliationUniversity of Greifswald, Institute of Microbiology
contact emailzuehlke@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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