PXD041883
PXD041883 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | mARC1 knockdown protects liver from diet-induced NASH in mouse |
Description | Human genetic studies have identified several MARC1 variants as protective against non-alcoholic fatty liver diseases (NAFLD). The MARC1 variants are associated with reduced lipid profiles, liver enzymes, and liver-related mortality. However, the role of mitochondrial amidoxime reducing component 1 (mARC1), encoded by MARC1, in NAFLD is still unknown and the therapeutic potential of this target has never been developed. Given that mARC1 is mainly expressed in hepatocytes, we developed an N-acetylgalactosamine conjugated mouse mARC1 siRNA to address this. In ob/ob mice, knockdown of mARC1 in mouse hepatocytes resulted in decreased liver weight, serum lipid enzymes, low-density lipoprotein cholesterol, and liver triglycerides. Loss of mARC1 also improved the lipid profiles and attenuated liver pathological changes in two diet-induced nonalcoholic steatohepatitis (NASH) mouse models. A comprehensive analysis of mARC1-deficient liver in NASH by metabolomics, proteomics, and lipidomics showed that mARC1 knockdown partially restored metabolites and lipids altered by diets. Taken together, loss of mARC1 protects mouse liver from NASH, suggesting a potential therapeutic approach of NASH by downregulation of mARC1 in hepatocytes. |
HostingRepository | MassIVE |
AnnounceDate | 2024-05-09 |
AnnouncementXML | Submission_2024-05-09_13:21:08.430.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matt Rardin |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-04-28 15:37:33 | ID requested | |
⏵ 1 | 2024-05-09 13:21:08 | announced |
Publication List
no publication |
Keyword List
submitter keyword: MARC1, NASH, TMT |
Contact List
Matthew Rardin | |
---|---|
contact affiliation | Amgen |
contact email | mrardin@amgen.com |
lab head | |
Matt Rardin | |
contact affiliation | Amgen |
contact email | mrardin@amgen.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091820/ |