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PXD041858

PXD041858 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLight Activated BioID (LAB): an optically activated proximity labeling system to study protein-protein interactions
DescriptionProximity labeling with genetically encoded enzymes is widely used to study protein-protein interactions in cells. However, the resolution and accuracy of proximity labeling methods are limited by a lack of control over the enzymatic labeling process. Here, we present a high spatial and temporal resolution technology that can be activated on demand using light, for high accuracy proximity labeling. Our system, called Light Activated BioID (LAB), is generated by fusing the two halves of the split-TurboID proximity labeling enzyme to the photodimeric proteins CRY2 and CIB1. Using live cell imaging, immunofluorescence, western blotting, and mass spectrometry, we show that upon exposure to blue light, CRY2 and CIB1 dimerize, reconstitute the split-TurboID enzyme, and biotinylate proximate proteins. Turning off the light halts the biotinylation reaction. We validate LAB in different cell types and demonstrate that it can identify known binding partners of proteins while reducing background labeling and false positives.
HostingRepositoryMassIVE
AnnounceDate2023-10-02
AnnouncementXMLSubmission_2023-10-02_13:59:22.211.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmittergabriela grigorean
SpeciesList scientific name: Canis lupus familiaris; common name: dog; NCBI TaxID: 9615; scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; scientific name: Aequorea victoria; NCBI TaxID: 6100;
ModificationListOxidation; Acetyl; Deamidated
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-27 13:51:57ID requested
12023-10-02 13:59:22announced
Publication List
no publication
Keyword List
submitter keyword: TurboID, BioID, E-cadherin, Proximity labeling, Light-activated proximity labeling, Split TurboID
Contact List
Sanjeevi Sivasankar
contact affiliationUniversity of California, Davis
contact emailssivasankar@ucdavis.edu
lab head
gabriela grigorean
contact affiliationucd
contact emailggrigorean@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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