PXD041551 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of two novel proteins involved in mitochondrial DNA anchoring in Trypanosoma brucei |
Description | Trypanosoma brucei is a single celled eukaryotic parasite in the group of the Kinetoplastea. The parasite harbors a single mitochondrion with a singular mitochondrial genome that is known as the kinetoplast DNA (kDNA). The kDNA consists of a unique network of thousands of interlocked circular DNA molecules. To ensure proper inheritance of the kDNA to the daughter cells, the genome is physically linked to the basal body, the master organizer of the cell cycle in trypanosomes. The connection that spans, cytoplasm, mitochondrial membranes and the mitochondrial matrix is mediated by the Tripartite Attachment Complex (TAC). Using a combination of proteomics and RNAi we test the current model of hierarchical TAC assembly and identify TbmtHMG44 and TbKAP68 as novel candidates of a complex that connects the TAC to the kDNA. Depletion of TbmtHMG44 or TbKAP68 each leads to a strong kDNA loss but not missegregation phenotype as previously defined for TAC components. We demonstrate that the proteins rely on both the TAC and the kDNA for stable localization to the interface between these two structures. In vitro experiments suggest a direct interaction between TbmtHMG44 and TbKAP68 and that recombinant TbKAP68 is a DNA binding protein. We thus propose that TbmtHMG44 and TbKAP68 are part of a distinct complex connecting the kDNA to the TAC. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:50:33.924.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | F Butter |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-14 12:16:43 | ID requested | |
1 | 2023-06-23 11:30:39 | announced | |
⏵ 2 | 2024-10-22 05:50:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MS/MS |
Contact List
Falk Butter |
contact affiliation | Institute of Molecular Biology (IMB) Ackermannweg 4, 55128 Mainz |
contact email | f.butter@imb.de |
lab head | |
F Butter |
contact affiliation | Quantitative Proteomics Institute of Molecular Biology (IMB) |
contact email | f.butter@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041551
- Label: PRIDE project
- Name: Characterization of two novel proteins involved in mitochondrial DNA anchoring in Trypanosoma brucei