PXD041296 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional viromic screens uncover regulatory RNA elements |
Description | The number of sequenced viral genomes has surged recently, presenting an opportunity to understand viral diversity and uncover unknown regulatory mechanisms. Here we conducted a screening of 30,367 viral segments from 143 species representing 96 genera and 37 families. Using a library of viral segments in 3′ UTR, we identified hundreds of elements impacting RNA abundance, translation, and nucleocytoplasmic distribution. To illustrate the power of this approach, we investigated K5, an element conserved in kobuviruses, and found its potent ability to enhance mRNA stability and translation in various contexts, including adeno-associated viral vectors and synthetic mRNAs. Moreover, we identified a previously uncharacterized protein, ZCCHC2, as a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. This study provides a unique resource for virus and RNA research and highlights the potential of the virosphere for biological discoveries.
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HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:47:25.748.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jenny Seo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-03 00:18:34 | ID requested | |
1 | 2023-06-23 01:53:09 | announced | |
⏵ 2 | 2023-11-14 06:47:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, RAPID, LC-MS/MS |
Contact List
V. Narry Kim |
contact affiliation | School of Biological Sciences, Seoul National University Center for RNA Research, Institute for Basic Science |
contact email | narrykim@snu.ac.kr |
lab head | |
Jenny Seo |
contact affiliation | School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea. |
contact email | rjenny204@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD041296
- Label: PRIDE project
- Name: Functional viromic screens uncover regulatory RNA elements