PXD041295

PXD041295 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Rapid and Highly-Efficient Purification of Extracellular Vesicles Enabled by TiO2 hybridized Spongy-like Polymer
Description	We developed a novel purification medium of extracellular vesicles by constructing spongy-like monolithic polymer kneaded with TiO2 microparticles (TiO2-hybridized spongy monolith, TiO2-SPM). TiO2-SPM was applied in a solid-phase extraction format and enabled simple, rapid and highly-efficient purification of EVs. This is due to the high permeability caused by continuous large flow-through pores of the monolithic skeleton (median pore size; 5.21 μm) and the specific interaction of embedded TiO2 with phospholipids of the lipid bilayers. Our method also excels in efficiency, collecting small-EVs (SEVs) from the same volume of a cell culture medium 130.7 times more than typical ultracentrifugation. The purification method was completed within 1 hour with simple operations and was directly applied to serum SEVs. Finally, we demonstrated flexibility toward shape and size of our method by depleting EVs from fetal bovine serum (FBS), which is a necessary process to prevent contamination of culture cell-derived EVs with exogenous FBS-derived EVs. Our method will eliminate tedious and difficult purification processes of EVs, providing a universal purification platform for EV-based drug discovery and pathological diagnosis.
HostingRepository	jPOST
AnnounceDate	2024-08-01
AnnouncementXML	Submission_2024-07-31_08:00:05.346.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eisuke Kanao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-02 22:54:25	ID requested
⏵ 1	2024-07-31 08:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: extracellular vesicle, TiO2, spongy-like polymer, purification method, solid phase extraction

submitter keyword: extracellular vesicle, TiO2, spongy-like polymer, purification method, solid phase extraction

Contact List

Eisuke Kanao
lab head
Eisuke Kanao
contact affiliation	Graduate School of Pharmaceutical Sciences, Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002111/