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PXD041229

PXD041229 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe genetic and dietary landscape of the muscle insulin signalling network
DescriptionUsing mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in the skeletal muscle of five genetically distinct inbred mouse strains exposed to two controlled dietary environments, with and without acute insulin treatment. Almost half of the insulin-regulated phosphoproteome was altered by genetic background independently of diet, and high-fat high-sugar feeding also affected insulin signalling in a strain-dependent manner. Our data illuminated signalling network organisation principles, including the uncoupling of phosphosites targeted by the same kinase. Associating diverse signalling responses with insulin-stimulated glucose uptake uncovered regulators of muscle insulin responsiveness, including the regulatory phosphosite S469 on Pfkfb2, a key glycolytic enzyme.
HostingRepositoryPRIDE
AnnounceDate2023-12-21
AnnouncementXMLSubmission_2023-12-21_04:29:18.158.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSean Humphrey
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-30 21:20:58ID requested
12023-12-21 04:29:18announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phosphoproteomics, glucose regulation, phosphoproteome, diet, environment, genetic, skeletal muscle, mouse
Contact List
Sean Humphrey
contact affiliationFunctional Phosphoproteomics, Murdoch Children's Research Institute, Australia
contact emailsean.humphrey@mcri.edu.au
lab head
Sean Humphrey
contact affiliationMurdoch Children's Research Institute
contact emailsean.j.humphrey@gmail.com
dataset submitter
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Dataset FTP location
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