PXD041229 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The genetic and dietary landscape of the muscle insulin signalling network |
Description | Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in the skeletal muscle of five genetically distinct inbred mouse strains exposed to two controlled dietary environments, with and without acute insulin treatment. Almost half of the insulin-regulated phosphoproteome was altered by genetic background independently of diet, and high-fat high-sugar feeding also affected insulin signalling in a strain-dependent manner. Our data illuminated signalling network organisation principles, including the uncoupling of phosphosites targeted by the same kinase. Associating diverse signalling responses with insulin-stimulated glucose uptake uncovered regulators of muscle insulin responsiveness, including the regulatory phosphosite S469 on Pfkfb2, a key glycolytic enzyme. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-21 |
AnnouncementXML | Submission_2023-12-21_04:29:18.158.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sean Humphrey |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-30 21:20:58 | ID requested | |
⏵ 1 | 2023-12-21 04:29:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phosphoproteomics, glucose regulation, phosphoproteome, diet, environment, genetic, skeletal muscle, mouse |
Contact List
Sean Humphrey |
contact affiliation | Functional Phosphoproteomics, Murdoch Children's Research Institute, Australia |
contact email | sean.humphrey@mcri.edu.au |
lab head | |
Sean Humphrey |
contact affiliation | Murdoch Children's Research Institute |
contact email | sean.j.humphrey@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041229
- Label: PRIDE project
- Name: The genetic and dietary landscape of the muscle insulin signalling network