PXD041188

PXD041188 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based proteomic landscape of rice reveals a post-transcriptional regulatory role of N6-methyladenosine
Description	Rice is one of the most important staple food and model species in plant biology, yet the quantitative proteomes of rice are largely uncharacterized. Here we quantify the relative protein levels of over 15,000 genes across rice major tissues using a tandem mass tag strategy followed by intensive fractionation and mass spectrometry. We identify tissue-specific or tissue-enriched proteins related to functional specificity of individual tissues. Proteogenomic comparison between rice and Arabidopsis reveals conserved proteome expression, which is different from mammals that strong separation of species rather than tissues. Notably, profiling of N6-methyladenosine (m6A) across the rice major tissues shows that m6A at untranslated regions is negatively correlated with protein abundance and contributes to the discordance of RNA and protein levels. We also demonstrate that our data are valuable to identify novel genes required for regulating m6A methylation. Taken together, this study provides a paradigm for in-depth proteogenomic studies of rice.
HostingRepository	PRIDE
AnnounceDate	2024-05-01
AnnouncementXML	Submission_2024-04-30_23:04:52.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Liang
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-29 08:16:29	ID requested
⏵ 1	2024-04-30 23:04:53	announced

Dataset with its publication pending

submitter keyword: Rice proteomic

Liang zhe
contact affiliation	Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
contact email	liangzhe@caas.cn
lab head
Zhe Liang
contact affiliation	Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
contact email	liangzhe@caas.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041188
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based proteomic landscape of rice reveals a post-transcriptional regulatory role of N6-methyladenosine