PXD041085

PXD041085 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mapping adipocyte interactome networks by HaloTag-enrichment–mass spectrometry
Description	Mapping protein interaction complexes in their natural state in a cell in vivo is a holy grail of protein network analysis. We here describe the development of HaloMS, a high-throughput HaloTag-based affinity purification–mass spectrometry assay for protein interaction discovery. The approach enabled quick capture of newly expressed protein and eliminated the tedious conventional one-by-one assay. As a proof-of-principle, we used HaloMS to evaluate protein complex interactions of 17 regulatory proteins in human adipocyte. The adipocyte interactome network was validated using an in vitro pull-down assay and artificial intelligence-based prediction tools. The application of HaloMS to probe adipocyte differentiation signaling pathways allowed the identification of previously unknown transcription factor–protein complexes and revealed proteome-wide human adipocyte transcription factor networks. This study using new technology reveals hidden networks of adipocyte signaling pathways, shedding light on how signals from different pathways are integrated.
HostingRepository	jPOST
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-21_08:00:20.566.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junshi Yazaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification
Instrument	Q Exactive; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-24 02:07:38	ID requested
⏵ 1	2024-05-21 08:00:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: adipocyte, interactome, cell differentiation, transcription factor, high-throughput affinity purification, mass spectrometry, artificial-intelligence prediction, AlphaFold2, systems biology, heterogeneous nuclear ribonucleoprotein

submitter keyword: adipocyte, interactome, cell differentiation, transcription factor, high-throughput affinity purification, mass spectrometry, artificial-intelligence prediction, AlphaFold2, systems biology, heterogeneous nuclear ribonucleoprotein

Contact List

Junshi Yazaki
lab head
Junshi Yazaki
contact affiliation	RIKEN
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002092/