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PXD040957

PXD040957 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of immune cells from liver tumors reveals immunotherapy targets
DescriptionElucidating the mechanisms by which immune cells become dysfunctional in tumors is critical to developing next-generation immunotherapies. We profiled proteomes of cancer cells, monocyte/macrophages, CD4+ and CD8+ T cells, and NK cells isolated from tumors, liver, and blood of 48 patients with hepatocellular carcinoma. We found that tumor macrophages induce the sphingosine-1-phospate-degrading enzyme SGPL1, which dampened their inflammatory phenotype and anti-tumor function in vivo. We further discovered that the signaling scaffold protein AFAP1L2, typically only found in activated NK cells, is also upregulated in chronically stimulated CD8+ T cells in tumors. Ablation of AFAP1L2 in CD8+ T cells increased their viability upon repeated stimulation and enhanced their antitumor activity synergistically with PD-L1 blockade in mouse models. Our data revealed new targets for immunotherapy and provide a resource on immune cell proteomes in liver cancer (www.immunomics.ch/liver).
HostingRepositoryPRIDE
AnnounceDate2023-04-20
AnnouncementXMLSubmission_2023-04-19_16:20:32.668.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatteoPecoraro
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-17 16:09:03ID requested
12023-04-19 16:20:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: NK cells, macrophages, immunotherapy, CD8,Hepatocellular carcinoma, T cells, CD4, monocytes
Contact List
RogerGeiger
contact affiliationInstitute for Research in Biomedicine (IRB), Università della Svizzera italiana, Bellinzona, Switzerland
contact emailroger.geiger@irb.usi.ch
lab head
MatteoPecoraro
contact affiliationInstitute for Research in Biomedicine, Via Vincenzo Vela 6 - CH-6500 Bellinzona
contact emailmatteo.pecoraro@irb.usi.ch
dataset submitter
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Dataset FTP location
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