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PXD040947

PXD040947 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExpanding the Characterization of Microbial Ecosystems using DIA-PASEF Metaproteomics
DescriptionMetaproteomics is gaining momentum in microbiome research due to the multi-dimensional information it provides. However, current approaches have reached their detection limits. We present a highly-sensitive metaproteomic workflow using the extra information captured by Parallel Accumulation-Serial Fragmentation (PASEF) technology. The comparison of different acquisition modes and data analysis software packages showed that DIA-PASEF and DIA-NN doubled protein identifications in the mouse gut microbiota and, importantly, also in the host proteome compared to DDA-PASEF. DIA-PASEF significantly improved peptide detection reproducibility and quantification accuracy, which resulted in more than twofold identified taxa, reaching depths comparable to metagenomic studies. Consequently, DIA-PASEF exhibited improved coverage of functional networks revealing 131 additional pathways compared to DDA-PASEF. We applied our optimized workflow to a pre-clinical mouse model of chronic pain, in which we deciphered novel host-microbiome interactions. In summary, we present here a metaproteomic approach that paves the way for increasing the functional characterization of microbiome ecosystems and is applicable to diverse fields of biological research.
HostingRepositoryPRIDE
AnnounceDate2023-05-04
AnnouncementXMLSubmission_2023-05-04_01:51:42.605.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFengXian
SpeciesList scientific name: Microbiota; NCBI TaxID: 13613;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-17 09:22:41ID requested
12023-05-04 01:51:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Metaproteomics, DIA-PASEF, Mouse, Chronic pain, Gut microbiome
Contact List
ManuelaSchmidt
contact affiliationDivision of Pharmacology & Toxicology, Department of Pharmaceutical Sciences, University of Vienna
contact emailmanuela_schmidt@univie.ac.at
lab head
FengXian
contact affiliationDivision of Pharmacology and Toxicology, Department of Pharmaceutical Sciences, University of Vienna
contact emailfeng.xian@univie.ac.at
dataset submitter
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Dataset FTP location
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