PXD040900

PXD040900 is an original dataset announced via ProteomeXchange.

Title	Initiation of fibronectin fibrillogenesis is an enzyme-dependent process
Description	Fibronectin fibrillogenesis and mechanosensing both depend on integrin-mediated force transmission to the extracellular-matrix. However, force transmission is in itself dependent on fibrillogenesis, and fibronectin fibrils are found in soft embryos where high forces cannot be applied, suggesting that force cannot be the sole initiator of fibrillogenesis. Here we identify a novel nucleation step prior to force transmission, driven by fibronectin oxidation mediated by lysyl-oxidase enzyme family members. This oxidation induces fibronectin clustering that promotes early adhesion, alters cellular response to soft matrices, and enhances force transmission to the matrix. In contrast, absence of fibronectin oxidation abrogates fibrillogenesis, perturbs cell-matrix adhesion, and compromises mechanosensation. Moreover, fibronectin oxidation promotes cancer cells colony formation in soft agar as well as collective and single-cell migration. These results reveal a yet unidentified, force-independent enzyme-dependent mechanism that initiates fibronectin fibrillogenesis, establishing a critical step in cell adhesion and mechanosensing.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:36.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haguy Wolfenson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 13:05:57	ID requested
1	2023-04-17 06:23:05	announced
⏵ 2	2023-11-14 09:00:37	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: fibronectin, oxidation, lysyl oxidases

Haguy Wolfenson
contact affiliation	Department of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion – Israel Institute of Technology
contact email	haguyw@technion.ac.il
lab head
Haguy Wolfenson
contact affiliation	Technion - Israel Institute of Technology
contact email	haguyw@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040900
     1. Label: PRIDE project
     2. Name: Initiation of fibronectin fibrillogenesis is an enzyme-dependent process