PXD040882

PXD040882 is an original dataset announced via ProteomeXchange.

Title	Cell cycle-dependent gene networks for cell proliferation activated by nuclear CK2 complexes
Description	Protein kinase CK2 is a conserved serine/threonine kinase that participates in various cellular processes. Elevated nuclear expression of CK2α has been observed in human carcinomas, but mechanisms for its variable localization in cells are poorly understood. This study demonstrates a functional connection between nuclear CK2 and gene expression in relation to cell proliferation. Growth stimulation of quiescent human fibroblasts identified a pool of CK2 highly phosphorylated at serine 7 of the α subunit that translocated into the nucleus. This phosphorylation appeared essential for its nuclear localization and catalytic activity. Protein signatures associated with nuclear CK2 complexes revealed enrichment of transcription factors and chromatin remodelers that seemed unique during progression through G1 phase of the cell cycle. Chromatin immunoprecipitation-sequencing profiling demonstrated recruitment of CK2α to the active gene locus, more abundantly in late G1 phase than in early G1. Notably, we confirmed the presence of CK2α at transcriptional start sites of core histone genes, growth stimulus-associated genes, and ribosomal RNAs. This study suggests that nuclear CK2α complexes, uniquely constituted during cell cycle progression, are essential for cell proliferation, by activating histone genes, triggering ribosome biogenesis, and then synthesize the components necessary for cell division, specified in the nucleus and also in the nucleolus.
HostingRepository	jPOST
AnnounceDate	2023-10-22
AnnouncementXML	Submission_2023-10-21_10:34:51.037.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takeshi Shibata
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine
Instrument	TripleTOF 5600; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-14 23:14:05	ID requested
⏵ 1	2023-10-21 10:34:51	announced

Dataset with its publication pending

submitter keyword: CK2, cell cycle, ChIP-Seq, ChIP-qPCR, gene activation, growth-associated genes, histone genes, nano-LC mass spectrometry, nuclear localization, transcription

Miwako Kato Homma
lab head
Takeshi Shibata
contact affiliation	Sciex
dataset submitter

jPOST dataset URI

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