PXD040824 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Subcellular proteomics for determining iron limited remodeling of plastids in the model diatom Thalassiosira pseudonana |
Description | Diatoms are important primary producers in the world’s oceans, yet their growth is constrained in large regions by low bioavailable iron (Fe). Low Fe-induced limitation of primary production is due to requirements for Fe in components of essential metabolic pathways including photosynthesis and other chloroplast plastid functions. Studies have shown that under Fe-limited stress, diatoms alter plastid-specific processes, including components of electron transport. These physiological changes suggest changes of protein content and their abundance within the diatom plastid. While in-silico predictions provide putative information on plastid-localized proteins, knowledge of diatom plastid proteins remains limited in comparison to model photosynthetic organisms. To characterize proteins enriched in diatom plastids we have used shotgun proteomics to assess the proteome of subcellular plastid-enriched fractions from Thalassiosira pseudonana. To improve our understanding of how the plastid proteome is remodeled in response to Fe limitation, proteome sequencing has been performed on T. pseudonana grown under Fe replete and limited conditions. These analyses have shown that Fe limitation regulates major metabolic pathways in the plastid, including the Calvin cycle, as well as changes in light harvesting protein expression. In-silico localization predictions of proteins identified in this plastid-enriched proteome allowed for an in-depth comparison of theoretical vs observed plastid-localization, providing evidence for the potential of additional protein import pathways into the diatom plastid. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:59:49.643.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Brook Nunn |
SpeciesList | scientific name: Thalassiosira pseudonana CCMP1335; NCBI TaxID: 296543; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-13 11:01:04 | ID requested | |
1 | 2023-08-04 10:08:39 | announced | |
⏵ 2 | 2024-10-22 05:59:50 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: plastid, organelle, iron limitation, diatom, ocean |
Contact List
Brook Nunn |
contact affiliation | University of Washington Department of Genome Sciences |
contact email | brookh@uw.edu |
lab head | |
Brook Nunn |
contact affiliation | University of Washington |
contact email | brookh@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040824
- Label: PRIDE project
- Name: Subcellular proteomics for determining iron limited remodeling of plastids in the model diatom Thalassiosira pseudonana