PXD040584

PXD040584 is an original dataset announced via ProteomeXchange.

Title	Boundary of Life and Death: changes in mitochondrial and cytosolic proteomes of Arabidopsis thaliana suspension cells associated with programmed cell death
Description	Despite the critical role of programmed cell death (PCD) in plant development and defence responses, its regulation is not fully understood. It has been proposed that mitochondria may control the early stages of plant PCD, but the details of this regulation are currently unknown. Here, we used Arabidopsis thaliana cell suspension culture, a model system that enables induction and precise monitoring of PCD rates, as well as chemical manipulation of this process to generate a quantitative profile of the alterations in mitochondrial and cytosolic proteomes associated with early stages of plant PCD induced by heat stress. The cells were subjected to PCD-inducing heat levels (10 min, 54oC), with/without the calcium channel inhibitor and PCD blocker LaCl3. The stress treatment was followed by separation of cytosolic and mitochondrial fractions and mass spectrometry-based proteome analysis. Heat stress induced rapid and extensive changes in protein abundance in both fractions and 113 mitochondrial proteins were detected in the cytosol upon PCD induction. Western blot analysis confirmed the release of mitochondrial heat shock protein 60 family members occurring in response to PCD-inducing, but not sublethal level of heat stress, that may indicate their cytosolic role as positive regulators of PCD in plants. In our system, LaCl3 appeared to act downstream of cell death initiation signal, as it did not affect the release of mitochondrial proteins, but instead partially inhibited changes occurring in the cytosolic fraction, including upregulation of proteins with hydrolytic activity. Collectively, these generated data provide new insights into the regulation of cell death and survival decisions in plant cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:51:35.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-03 09:27:27	ID requested
1	2023-07-01 05:16:59	announced
⏵ 2	2024-10-22 05:51:36	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: cytosol, proteomics, mitochondria,plant programmed cell death, heat stress

Dr Jim Carolan
contact affiliation	Department of Biology, Maynooth University, Co. Kildare,Ireland
contact email	james.carolan@nuim.ie
lab head
James Carolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040584
     1. Label: PRIDE project
     2. Name: Boundary of Life and Death: changes in mitochondrial and cytosolic proteomes of Arabidopsis thaliana suspension cells associated with programmed cell death