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PXD040435

PXD040435 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Plant Unique ESCRT Component FREE1 Regulates Autophagosome Closure
DescriptionThe energy sensor AMP-activated protein kinase (AMPK) can activate autophagy when cellular energy production becomes compromised. However, the degree to which nutrient sensing impinges on the autophagosome closure remains unknown. Here, we provide the mechanism underlying a plant unique protein FREE1, upon autophagy-induced SnRK1α1-mediated phosphorylation, functions as a linkage between ATG conjugation system and ESCRT machinery to regulate the autophagosome closure upon nutrient deprivation. Using high-resolution microscopy, 3D-electron tomography, and protease protection assay, we showed that unclosed autophagosomes accumulated in free1 mutants. Proteomic, cellular and biochemical analysis revealed the mechanistic connection between FREE1 and the ATG conjugation system/ESCRT-III complex in regulating autophagosome closure. Mass spectrometry analysis showed that the evolutionary conserved plant energy sensor SnRK1α1 phosphorylates FREE1 and recruits it to the autophagosomes to promote closure. Mutagenesis of the phosphorylation site on FREE1 caused the autophagosome closure failure. Our findings unveil how cellular energy sensing pathways regulate autophagosome closure to maintain cellular homeostasis.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:00:22.421.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLei Feng
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-27 06:00:39ID requested
12023-11-14 09:00:22announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: AP-MS, Autophagosome, Arabidopsis thaliana,ESCRT, FREE1
Contact List
Lei Feng
contact affiliationThe Chinese University of Hong Kong
contact emaillfeng@link.cuhk.edu.hk
lab head
Lei Feng
contact affiliationThe Chinese University of Hong Kong
contact emaillfeng@link.cuhk.edu.hk
dataset submitter
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Dataset FTP location
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