PXD040435 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Plant Unique ESCRT Component FREE1 Regulates Autophagosome Closure |
Description | The energy sensor AMP-activated protein kinase (AMPK) can activate autophagy when cellular energy production becomes compromised. However, the degree to which nutrient sensing impinges on the autophagosome closure remains unknown. Here, we provide the mechanism underlying a plant unique protein FREE1, upon autophagy-induced SnRK1α1-mediated phosphorylation, functions as a linkage between ATG conjugation system and ESCRT machinery to regulate the autophagosome closure upon nutrient deprivation. Using high-resolution microscopy, 3D-electron tomography, and protease protection assay, we showed that unclosed autophagosomes accumulated in free1 mutants. Proteomic, cellular and biochemical analysis revealed the mechanistic connection between FREE1 and the ATG conjugation system/ESCRT-III complex in regulating autophagosome closure. Mass spectrometry analysis showed that the evolutionary conserved plant energy sensor SnRK1α1 phosphorylates FREE1 and recruits it to the autophagosomes to promote closure. Mutagenesis of the phosphorylation site on FREE1 caused the autophagosome closure failure. Our findings unveil how cellular energy sensing pathways regulate autophagosome closure to maintain cellular homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:00:22.421.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lei Feng |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-27 06:00:39 | ID requested | |
⏵ 1 | 2023-11-14 09:00:22 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: AP-MS, Autophagosome, Arabidopsis thaliana,ESCRT, FREE1 |
Contact List
Lei Feng |
contact affiliation | The Chinese University of Hong Kong |
contact email | lfeng@link.cuhk.edu.hk |
lab head | |
Lei Feng |
contact affiliation | The Chinese University of Hong Kong |
contact email | lfeng@link.cuhk.edu.hk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD040435 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040435
- Label: PRIDE project
- Name: The Plant Unique ESCRT Component FREE1 Regulates Autophagosome Closure