PXD040409

PXD040409 is an original dataset announced via ProteomeXchange.

Title	BmATAD3A mediates mitochondrial ribosomal protein expression to maintain the mitochondrial energy metabolism of the silkworm Bombyx mori
Description	ATAD3A is a mitochondrial membrane protein in the ATPase family containing an AAA+ domain, which is widely involved in mitochondrial metabolism, protein transport, cell growth, development, and other important life processes. It has previously been reported that the deletion of ATAD3A causes individual growth and development defects in humans, mice, and Caenorhabditis elegans. To further analyze the mechanism of ATAD3A defects affecting individual development, we constructed a BmATAD3A defect model in silkworm larvae to provide a reference for ATAD3A genetic diseases and molecular regulation mechanisms. The results showed that knockout of the BmATAD3A gene could significantly affect individual weight, survival rate, ATPase production, and mitochondrial metabolism after 24 h of individual incubation. Metabonomics and transcriptomics combined analysis further showed that knockout of BmATAD3A could inhibit individual amino acid biosynthesis by regulating mitochondrial ribosomal protein expression. Meanwhile, the knockout of BmATAD3A could block mitochondrial activity and ATPase synthesis through the mitochondrial ribosomal protein BmmRPL11 and thereby inhibit mitochondrial oxidative phosphorylation. These results could provide new insights into the inhibition of individual development caused by ATAD3A deficiency, thus allowing the identification of new directions for the targeted therapy of diseases caused by abnormal expression of ATAD3A.
HostingRepository	iProX
AnnounceDate	2023-02-27
AnnouncementXML	Submission_2023-04-02_02:43:34.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Wang
SpeciesList	scientific name: Bombyx mori; NCBI TaxID: 7091;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 00:00:13	ID requested
⏵ 1	2023-04-02 02:43:34	announced

Dataset with its publication pending

submitter keyword: BmATAD3A, Bombyx mori, Energy Metabolism, Untargeted Metabolomics

Minhui Pan
contact affiliation	Southwest University
contact email	pmh047@126.com
lab head
Jie Wang
contact affiliation	Southwest University
contact email	wangdd1105@163.com
dataset submitter

iProX dataset URI