PXD040207 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis |
Description | Deciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. A sophisticated network of transcription factors has been implicated in SCW synthesis in plants, but little is known about the implication of RNA-binding proteins in this process. Here we report that two RNA-binding proteins homologous to the animal translational regulator Musashi, Musashi-Like2/MSIL2 and Musashi-Like4/MSIL4, function redundantly to control SCW formation in interfascicular fibers and the setting of biomass recalcitrance. We show that the disruption of MSIL2/4 decreases the abundance of lignin in fibers and triggers an hypermethylation of glucuronoxylan that is linked to an over-accumulation of GlucuronoXylan Methyltransferase1/3 (GXM1/3) proteins. We demonstrate that MSIL4 binds to the GXM1/3 mRNAs, likely repressing their translation in wild-type plants. Our results reveal cell-type-specific mechanisms underlying SCW formation in Arabidopsis and point to a novel aspect of SCW regulation linking translational repression to regulation of glucuronoxylan methylation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-28 |
AnnouncementXML | Submission_2023-09-28_09:10:25.457.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD040207 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | LaurianeKuhn |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-16 14:54:02 | ID requested | |
⏵ 1 | 2023-09-28 09:10:25 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Arabidopsis, Musashi-type transcriptional regulator,cell wall, interactomic |
Contact List
DominiqueGagliardi |
contact affiliation | Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France |
contact email | dominique.gagliardi@ibmp-cnrs.unistra.fr |
lab head | |
LaurianeKuhn |
contact affiliation | CNRS, IBMC, FRC1589 |
contact email | l.kuhn@ibmc-cnrs.unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD040207 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040207
- Label: PRIDE project
- Name: Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis