PXD040191 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins |
Description | Acute lysosomal membrane damage reduces the cellular population of functional lysosomes. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in TFEB/TFE3-depleted cells. We combined proximity labelling based proteomics, biochemistry and high-resolution microscopy to unravel a new lysosomal membrane regeneration pathway which is dependent on ATG8, lysosomal membrane protein LIMP2, the Rab7 GAP TBC1D15, and proteins required for autophagic lysosomal reformation (ALR) including Dynamin2, Kinesin5B and Clathrin. Upon lysosomal damage, LIMP2 act as a lysophagy receptor to bind ATG8, which in turn recruits TBC1D15 to damaged membranes. TBC1D15 hydrolyses Rab7-GTP to segregate the damaged lysosomal mass and provides a scaffold to assemble and stabilize the ALR machinery, potentiating the formation of lysosomal tubulesand subsequent Dynamin2-dependent scission. TBC1D15-mediated lysosome regeneration was also observed in a cell culture model of oxalate nephropathy. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:00:36.777.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anshu Bhattacharya |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-16 14:02:55 | ID requested | |
1 | 2023-03-07 05:30:56 | announced | |
⏵ 2 | 2023-11-14 09:00:37 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: autophagy, lysosomal regeneration,lysosomes, autophagic lysosomal reformation, TBC family of proteins |
Contact List
Ivan Dikic |
contact affiliation | Institute of Biochemistry II; University Hospital of Goethe University Frankfurt Building 75 ,Ground floor, Room 0.205 Theodor-Stern-Kai 7 60590 Frankfurt am Main, Germany |
contact email | dikic@biochem2.uni-frankfurt.de |
lab head | |
Anshu Bhattacharya |
contact affiliation | Goethe University, Institute of Biochemistry 2, Buchmann Institute For Molecular Life Sciences |
contact email | bhattacharya@med.uni-frankfurt.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040191
- Label: PRIDE project
- Name: A Lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins