PXD039931 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows |
Description | Macrocyclic peptides are attractive for chemoproteomic applications due to their modular synthesis and potential for high target selectivity. We describe a solid phase synthesis method for the efficient generation of libraries of small macrocycles that contain an electrophile and alkyne handle. The modular synthesis produces libraries that can be directly screened using simple SDS-PAGE readouts and then optimal lead molecules applied to proteomic analysis. We generated a library of 480 macrocyclic peptides containing the weakly reactive fluorosulfate (OSF) electrophile. Initial screening of a subset of the library containing each of the various diversity elements identified initial molecules of interest. The corresponding positional and confirmational isomers were then screened to select molecules that showed specific protein labeling patterns that were dependent on the probe structure. The most promising hits were applied to standard chemoproteomic workflows to identify protein targets. Our results demonstrate the feasibility of rapid, on-resin synthesis of diverse macrocyclic electrophiles to generate new classes of covalent ligands. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:24:27.204.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniel Abegg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-07 14:28:21 | ID requested | |
1 | 2023-03-02 16:11:57 | announced | |
⏵ 2 | 2023-11-14 08:24:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SuFEx, chemoproteomics, electrophile, macrocycles,Fluorosulfate |
Contact List
Dr. Alexander Adibekian |
contact affiliation | Department of Chemistry University of Illinois at Chicago 845 West Taylor Street - Room 1454 SES Chicago, Illinois, 60607, USA |
contact email | aadibeki@uic.edu |
lab head | |
Daniel Abegg |
contact affiliation | University of Illinois at Chicago |
contact email | dabegg@uic.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039931
- Label: PRIDE project
- Name: Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows