PXD039903
PXD039903 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Distinctive Interactomes of RNA polymerase II phosphorylation during different stages of transcription |
| Description | During eukaryotic transcription, RNA polymerase II undergoes dynamic post-translational modification on the C-terminal domain (CTD) of the largest subunit , generating a sophisticated PTM landscape for the spatiotemporal recruitment to transcriptional regulators. To delineate the protein interactomes recruited to Pol II at different stages of transcription, we in vitro reconstructed phosphorylation patterns of the CTD at Ser5 and Ser2 positions, the hallmark phosphorylation at the initation and productive elongation stages of transcription, respectively. Distinctive protein interactomes indicates different proteins are recruited to RNA polymerase II at different stages of transcription by the phosphorylation of Ser2 and Ser5 of the CTD heptads. Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP) specifically binds to the Ser2 of the heptad. The loss of the interaction between CHERP and Pol II results in broad alternative splicing events. Our method points to a new method to distinguish the PTM codes that coordinate the transcription process. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-02-06 |
| AnnouncementXML | Submission_2023-02-06_16:23:52.657.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brendan Floyd |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Oxidation |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-02-06 16:14:42 | ID requested | |
| ⏵ 1 | 2023-02-06 16:23:53 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: RNA polymerase II, phosphorylation, pulldown |
Contact List
| Edward Marcotte | |
|---|---|
| contact affiliation | University of Texas at Austin |
| contact email | marcotte@utexas.edu |
| lab head | |
| Brendan Floyd | |
| contact affiliation | University of Texas at Austin |
| contact email | bmfloyd@utexas.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091213/ |
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