PXD039865 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | EPLIN-Beta is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration |
Description | Polyamines promote cellular proliferation, and their levels are controlled by ornithine decarboxylase antizyme 1 (Az1), through the proteasome-mediated, ubiquitin-independent degradation of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis. Az1-mediated degradation of other substrates such as cyclin D1, DNp73 and Mps1 also regulate cell growth and centrosome amplification. Collectively, the currently known six Az1-substrates are all linked with tumorigenesis. To understand if Az1-mediated protein degradation might play a key role in regulating cellular process associated with tumorigenesis, we employed quantitative proteomics to identify novel Az1 substrates. Here, we describe the identification of LIM domain and Actin-binding protein 1 (LIMA1), also known as epithelial protein lost in neoplasm (EPLIN), as a new target of Az1. Interestingly, between the two isoforms of EPLIN-Alpha and -Beta, only EPLIN-Beta is the substrate of Az1, degraded in an ubiquitination-independent manner. Absence of Az1 led to elevated EPLIN-Beta levels, which caused enhanced cellular migration of Az1-/- cells. Consistently, higher levels of LIMA1 correlated with poorer overall survival of colorectal cancer patients. Taken together, this study identifies EPLIN-Beta as a novel Az1 substrate regulating cellular migration. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:47:17.049.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Suat Peng Neo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-04 13:57:34 | ID requested | |
1 | 2023-06-07 20:49:16 | announced | |
⏵ 2 | 2024-10-22 05:47:17 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ubiquitin-independent degradation,Antizyme, cell migration, EPLIN/LIMA1 |
Contact List
Jayantha Gunaratne |
contact affiliation | TBP Lab, Institute of Molecular & Cellular Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
contact email | jayanthag@imcb.a-star.edu.sg |
lab head | |
Suat Peng Neo |
contact affiliation | Institute of Molecular and Cell Biology (ASTAR) |
contact email | neosp@imcb.a-star.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039865
- Label: PRIDE project
- Name: EPLIN-Beta is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration