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PXD039865

PXD039865 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEPLIN-Beta is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration
DescriptionPolyamines promote cellular proliferation, and their levels are controlled by ornithine decarboxylase antizyme 1 (Az1), through the proteasome-mediated, ubiquitin-independent degradation of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis. Az1-mediated degradation of other substrates such as cyclin D1, DNp73 and Mps1 also regulate cell growth and centrosome amplification. Collectively, the currently known six Az1-substrates are all linked with tumorigenesis. To understand if Az1-mediated protein degradation might play a key role in regulating cellular process associated with tumorigenesis, we employed quantitative proteomics to identify novel Az1 substrates. Here, we describe the identification of LIM domain and Actin-binding protein 1 (LIMA1), also known as epithelial protein lost in neoplasm (EPLIN), as a new target of Az1. Interestingly, between the two isoforms of EPLIN-Alpha and -Beta, only EPLIN-Beta is the substrate of Az1, degraded in an ubiquitination-independent manner. Absence of Az1 led to elevated EPLIN-Beta levels, which caused enhanced cellular migration of Az1-/- cells. Consistently, higher levels of LIMA1 correlated with poorer overall survival of colorectal cancer patients. Taken together, this study identifies EPLIN-Beta as a novel Az1 substrate regulating cellular migration.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:47:17.049.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSuat Peng Neo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-04 13:57:34ID requested
12023-06-07 20:49:16announced
22024-10-22 05:47:17announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ubiquitin-independent degradation,Antizyme, cell migration, EPLIN/LIMA1
Contact List
Jayantha Gunaratne
contact affiliationTBP Lab, Institute of Molecular & Cellular Biology, Agency for Science, Technology and Research (A*STAR), Singapore
contact emailjayanthag@imcb.a-star.edu.sg
lab head
Suat Peng Neo
contact affiliationInstitute of Molecular and Cell Biology (ASTAR)
contact emailneosp@imcb.a-star.edu.sg
dataset submitter
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Dataset FTP location
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