PXD039806

PXD039806 is an original dataset announced via ProteomeXchange.

Title	Evaluation of the African swine fever virus-induced host shutoff using dynamic stable isotope labeling with amino acids in cell culture (SILAC)
Description	African swine fever is a viral disease of swine caused by the African swine fever virus (ASFV). Currently, ASFV is a serious threat to the global pig industry. A viral strategy to undermine host cell response is to establish a global shutoff of host protein synthesis (virus-induced shutoff, vhs). Here, we characterize ASFV-induced shutoff in primary porcine macrophages by measurement of relative protein synthesis rates based on stable isotope labeling with amino acids in cell culture (SILAC). The impact of ASFV infection on the synthesis of >2000 individual host proteins showed a high degree of variability ranging from complete shutoff to a strong induction of proteins that are absent from naïve cells. By GO-term enrichment analysis the cellular pathways that were most efficiently impacted by vhs were identified. The experimental setup is suitable to quantify vhs after infections with different viruses.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:38:26.199.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Axel Karger
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-02 08:32:10	ID requested
1	2023-05-31 02:23:24	announced
⏵ 2	2023-11-14 06:38:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: SILAC,African swine fever virus, virus host shutoff, VHS

Axel Karger
contact affiliation	Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut
contact email	axel.karger@fli.de
lab head
Axel Karger
contact affiliation	Friedrich-Loeffler-Institut
contact email	axel.karger@fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039806
     1. Label: PRIDE project
     2. Name: Evaluation of the African swine fever virus-induced host shutoff using dynamic stable isotope labeling with amino acids in cell culture (SILAC)