PXD039664 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes |
Description | Migrasomes are newly discovered cell organelles forming by local swelling of retraction fibers. The migrasome formation critically depends on tetraspanin proteins present in the retraction fiber membranes and is modulated by the membrane tension and bending rigidity. It remained unknown how and in which time sequence these factors are involved in migrasome nucleation, growth, and stabilization, and what are the possible intermediate stages of migrasome biogenesis. Here using live cell imaging and biomimetic system for migrasomes and retraction fibers, we reveal that migrasome formation is a two-stage process. At the first stage, which in biomimetic system is mediated by membrane tension, local swellings largely devoid of tetraspanin 4 form on the retraction fibers. At the second stage, tetraspanin 4 molecules migrate toward and onto these swellings, which grow up to several microns in size and transform into migrasomes. This tetraspanin 4 recruitment to the swellings is essential for migrasome growth and stabilization. Based on these findings we propose that the major role of tetraspanin proteins is in stabilizing the migrasome structure while the migrasome nucleation and initial growth stages can be driven by membrane mechanical stresses. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-29 |
AnnouncementXML | Submission_2023-01-28_23:56:01.707.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | TamarZiv |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-25 08:16:57 | ID requested | |
⏵ 1 | 2023-01-28 23:56:02 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Migrasome,Tetraspanin 4 |
Contact List
RayaSorkin |
contact affiliation | School of Chemistry, Raymond & Beverly Sackler Faculty of Exact Sciences and Center for Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv, Israel |
contact email | rsorkin@tauex.tau.ac.il |
lab head | |
TamarZiv |
contact affiliation | Technion |
contact email | tamarz@technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039664
- Label: PRIDE project
- Name: Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes