PXD039568

PXD039568 is an original dataset announced via ProteomeXchange.

Title	The effect of obesity on uterine receptivity is mediated by endometrial extracellular vesicles that control human endometrial stromal cell decidualization and trophoblast invasion
Description	The objectives of the present study were to determine whether obesity impacts human decidualization and the endometrial control of trophoblast invasion (both of which are required for embryo implantation) and evaluate the potential involvement of endometrial extracellular vesicles (EVs) in the regulation of these physiological processes. Using primary human cell cultures, we first demonstrated that obesity is associated with significantly lower in vitro decidualization of endometrial stromal cells (ESCs). We then showed that the trophoblastic cell line’s invasive ability was greater in the presence of conditioned media from cultures of ESCs from obese women. Using mass-spectrometry-based quantitative proteomics, we found that EVs isolated from uterine supernatants of biopsies from obese women (vs. nonobese women) presented a molecular signature focused on cell remodeling and angiogenesis. Lastly, the results of functional assays indicated that supplementation of the culture medium with EVs from nonobese women can rescue (at least in part) the defect in in vitro decidualization described in ESCs from obese women. Lastly, the addition of endometrial EVs from obese women (vs. nonobese women) was associated with significantly greater invasive activity by HTR-8/SVneo cells. In conclusion, our results provided new insights into the endometrial EVs’ pivotal role in the poor uterine receptivity observed in obese women.
HostingRepository	PRIDE
AnnounceDate	2023-10-09
AnnouncementXML	Submission_2023-10-09_04:55:06.254.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CélineHenry
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-20 02:22:48	ID requested
⏵ 1	2023-10-09 04:55:06	announced

Dataset with its publication pending

submitter keyword: vesicules, human, decidualization, proteomic

LaurentGalio
contact affiliation	INRAE-UMR 1198 BREED : Biologie de la Reproduction, Environnement, Epigénétique et Développement Equipe RHuMA : Reproduction Humaine et Modèles Animaux
contact email	laurent.galio@inrae.fr
lab head
CélineHenry
contact affiliation	INRAE
contact email	celine.henry@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039568
     1. Label: PRIDE project
     2. Name: The effect of obesity on uterine receptivity is mediated by endometrial extracellular vesicles that control human endometrial stromal cell decidualization and trophoblast invasion