PXD039527
PXD039527 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | PekUSZ_Development of Lysine Crotonyl-mimic Probe to Covalently Identify H3K27Cr Interacting Proteins |
Description | we developed a novel H3g27Cr peptide probe mimicking Histone lysine crotonylation, which could more effectively recognize Histone Kcr-interacting proteins through covalent proximity-induced bioconjugation. We initially validated the orthogonality and cysteine reactivity of crotonyl-like group through protein-ligand binding models of PDZΔRGS3 and PLCγ1-c SH2, and further showed the feasibility of H3g27Cr to covalently bind the known Kcr “eraser” HDAC1. With the H3g27Cr probe in hands, we successfully identified and validated one unknown H3K27Cr-interacting protein, the transcriptional activator STAT3, from AP-MS experiment in A549 cell lysates. The candidate protein STAT3 was found that directly bound to the Histone H3K27Ac/Cr in XL-MS and pull-down assays, and this interaction might reduce the H3K27Ac/Cr levels. |
HostingRepository | iProX |
AnnounceDate | 2023-01-17 |
AnnouncementXML | Submission_2023-01-17_03:38:44.012.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuhao An |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-01-17 03:38:32 | ID requested | |
⏵ 1 | 2023-01-17 03:38:44 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: H3g27Cr, Histone, lysine crotonylation, STAT3, HDAC1, AP-MS |
Contact List
Rui Wang | |
---|---|
contact affiliation | Pingshan Translational Medicine Center, Shenzhen Bay Laboratory |
contact email | wangrui@szbl.ac.cn |
lab head | |
Yuhao An | |
contact affiliation | Beijing Institute of Genomics, Chinese Academy of Sciences |
contact email | anchiyuhao@vip.qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |