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PXD039527

PXD039527 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePekUSZ_Development of Lysine Crotonyl-mimic Probe to Covalently Identify H3K27Cr Interacting Proteins
Descriptionwe developed a novel H3g27Cr peptide probe mimicking Histone lysine crotonylation, which could more effectively recognize Histone Kcr-interacting proteins through covalent proximity-induced bioconjugation. We initially validated the orthogonality and cysteine reactivity of crotonyl-like group through protein-ligand binding models of PDZΔRGS3 and PLCγ1-c SH2, and further showed the feasibility of H3g27Cr to covalently bind the known Kcr “eraser” HDAC1. With the H3g27Cr probe in hands, we successfully identified and validated one unknown H3K27Cr-interacting protein, the transcriptional activator STAT3, from AP-MS experiment in A549 cell lysates. The candidate protein STAT3 was found that directly bound to the Histone H3K27Ac/Cr in XL-MS and pull-down assays, and this interaction might reduce the H3K27Ac/Cr levels.
HostingRepositoryiProX
AnnounceDate2023-01-17
AnnouncementXMLSubmission_2023-01-17_03:38:44.012.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuhao An
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-17 03:38:32ID requested
12023-01-17 03:38:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: H3g27Cr, Histone, lysine crotonylation, STAT3, HDAC1, AP-MS
Contact List
Rui Wang
contact affiliationPingshan Translational Medicine Center, Shenzhen Bay Laboratory
contact emailwangrui@szbl.ac.cn
lab head
Yuhao An
contact affiliationBeijing Institute of Genomics, Chinese Academy of Sciences
contact emailanchiyuhao@vip.qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI