PXD039363
PXD039363 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Illuminating phenotypic drug responses of sarcoma cells to kinase inhibitors by phosphoproteomics |
| Description | Kinase inhibitors (KIs) are important cancer drugs but often display polypharmacology that is molecularly not understood. This disconnect is particularly apparent in cancer entities such as sarcomas for which the oncogenic drivers are often not clear. To investigate more systematically how the cellular proteotypes of sarcoma cells shape their response to molecularly targeted drugs, we profiled the proteomes and phosphoproteomes of 17 sarcoma cell lines and screened the same cells against 150 cancer drugs. The resulting 2,550 phenotypic drug profiles revealed distinct drug responses and the cellular activity landscapes derived from deep (phospho)proteomes (9-10,000 proteins and 10-27,000 phosphorylation sites per cell line) enabled several lines of analysis. For instance, connecting the (phospho)proteomic data with drug responses revealed known and novel mechanisms of action (MoAs) of kinase inhibitors and identified markers of drug sensitivity or resistance. All data is publically accessible via an interactive web application that enables exploration of this rich molecular resource for better understanding active signalling pathways in sarcoma cells, identifying treatment response predictors, and revealing novel MoA of clinical KIs. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-01-12 |
| AnnouncementXML | Submission_2023-01-12_01:52:15.971.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chien-Yun Lee |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | TMT6plex; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-01-11 07:52:15 | ID requested | |
| ⏵ 1 | 2023-01-12 01:52:16 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: proteotypes, phosphoproteomics, kinase inhibitor, sarcoma, drug response |
Contact List
| Bernhard Kuster | |
|---|---|
| contact affiliation | Chair of Proteomics and Bioanalytics, School of Life Sciences, Technical University of Munich |
| contact email | kuster@tum.de |
| lab head | |
| Chien-Yun Lee | |
| contact affiliation | Technical University of Munich |
| contact email | chienyun.lee@tum.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091043/ |
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