PXD039123

PXD039123 is an original dataset announced via ProteomeXchange.

Title	Verticillium dahliae Vta3 promotes ELV1 virulence factor gene expression in xylem sap, but tames Mtf1-mediated late stages of fungus-plant interactions and microsclerotia formation
Description	Verticillium transcription activator of adhesion 3 (Vta3) is required for plant root colonization and pathogenicity of the soil-borne vascular fungus Verticillium dahliae [Bui et al., 2018]. In this study, we discovered that Vta3 contributes to the virulence of V. dahliae on tomato plants by promoting ELV1 gene expression, which is not necessary for vegetative growth or production of conidiospores. Gene expression of the transcription factor Mtf1 is reduced in the presence of an intact VTA3 gene. The presence of an intact MTF1 gene triggers the expression of fungal effector genes and plant immune responses (transcription of tomato pathogenesis-related protein genes, and levels of pipecolic and salicylic acids functioning in tomato defense signaling against (hemi-) biotrophic pathogens), and is required for disease symptoms similar to those seen in tomato plants infected by the V. dahliae wild-type. Mtf1 promotes the formation of resting structures at the end of the infection cycle, which allows the fungus to survive in the soil and later to re-infect host plants. Protein pull-downs were performed with GFP-fused Vta3 to investigate whether there is evidence for a direct interaction of the regulators Vta3 and Mtf1. In this study, ribosomal proteins predominantly co-enriched with Vta3, and our data set did not give evidence that Mtf1 directly interacts with Vta3. Bui T-T, Harting R, Braus-Stromeyer SA, Tran V-T, Leonard M, Höfer A, et al. Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease. New Phytologist. 2019;221: 2138–2159. doi:10.1111/nph.15514
HostingRepository	PRIDE
AnnounceDate	2023-01-18
AnnouncementXML	Submission_2023-01-18_03:07:18.624.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	OliverValerius
SpeciesList	scientific name: Verticillium dahliae JR2; NCBI TaxID: 1202531;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 01:11:58	ID requested
⏵ 1	2023-01-18 03:07:19	announced

Dataset with its publication pending

submitter keyword: Verticillium dahliae, Master transcription factor 1, JR2, Mtf1, transcription factor, Vta3, Verticillium transcription activator of adhesion 3

Gerhard H.Braus
contact affiliation	Department of Molecular Microbiology and Genetics, Institute of Microbiology and Genetics and Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Germany
contact email	gbraus@gwdg.de
lab head
OliverValerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039123
     1. Label: PRIDE project
     2. Name: Verticillium dahliae Vta3 promotes ELV1 virulence factor gene expression in xylem sap, but tames Mtf1-mediated late stages of fungus-plant interactions and microsclerotia formation