PXD039121
PXD039121 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | L-lactate induced proteomic changes in rat hippocampus |
Description | Rats were anesthetized with sodium pentobarbital (50 mg/kg, i.p.) and placed in a stereotaxic instrument (Kopf instrument). After exposing the skull, bilateral craniotomy (0.5 - 0.8 mm holes, 4 mm posterior to bregma and 2.6 mm lateral from midline) was done. In one group of rats (Lactate group), each hippocampus (3.5 mm ventral from the surface of the skull at the craniotomy site) was infused with 100 nmol of L-lactate dissolved in 1µl of ACSF at a flow rate of 0.1 µl/min (controlled by microinjection pump; World Precision Instruments, USA). In another group of rats (ACSF group), 1 µl of artificial cerebrospinal fluid (ACSF; NaCl 124 mM, KCl 3 mM, CaCl2 2.4 mM, MgSO4 1.3 mM, glucose 10 mM, and HEPES 10 mM, pH = 7.3) was infused per HPC. Needle was kept at injection site for additional 5 minutes for the proper dispersion of solution after L-lactate/ACSF infusion. Then rats were kept into their cages for approximately 60 minutes. After that higher dose of sodium pentobarbital was given, and decapitation was done quickly. Following decapitation, the dura mater was removed carefully, and the brain was flipped out from skull into ice cold PBS using spatula. The right and left hippocampus were isolated on an ice-cold plate using sterile forceps and were stored immediately at -80°C until further use. Later, the right hippocampus was used as sample for mass spectrometry (MS). |
HostingRepository | jPOST |
AnnounceDate | 2023-02-23 |
AnnouncementXML | Submission_2023-02-22_20:58:47.665.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mastura Akter |
SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-12-30 21:09:37 | ID requested | |
⏵ 1 | 2023-02-22 20:58:48 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: lactate, hippocampus, proteomics, rat |
Contact List
Professor Ying Li | |
---|---|
lab head | |
Mastura Akter | |
contact affiliation | City University of Hong Kong |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001976/ |