PXD038964 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative phyloproteomics identifies conserved plasmodesmal proteins |
Description | Plasmodesmata are cytosolic bridges, lined by the plasma membrane and traversed by endoplasmic reticulum, connecting cells and tissues, and critical for many aspects of plant biology. While plasmodesmata are notoriously difficult to extract, tissue fractionation and proteomic analyses can yield valuable knowledge of their composition. Here we have generated two novel proteomes to expand tissue and taxonomic representation of plasmodesmata: one from mature Arabidopsis leaves and one from the moss Physcomitrium patens and leveraged these and existing data to perform a comparative analysis to identify evolutionarily conserved protein families that are associated with plasmodesmata. Thus, we identified b-1,3-glucanases, C2 lipid-binding proteins and tetraspanins as core plasmodesmal components that likely serve as essential structural or functional components. Our approach has not only identified elements of a conserved plasmodesmal proteome, but also demonstrated the added power offered by comparative analysis for recalcitrant samples. Conserved plasmodesmal proteins establish a basis upon which ancient plasmodesmal function can be further investigated to determine the essential roles these structures play in multicellular organism physiology in the green lineages. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:59:35.408.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthew Johnston |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-20 06:47:02 | ID requested | |
1 | 2023-01-13 02:01:16 | announced | |
⏵ 2 | 2023-11-14 08:59:35 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Arabidopsis,plasmodesmata, phyloproteomics, Physcomitrium |
Contact List
Christine Faulkner |
contact affiliation | Crop Genetics John Innes Centre Norfolk United Kingdom |
contact email | christine.faulkner@jic.ac.uk |
lab head | |
Matthew Johnston |
contact affiliation | Crop Genetics, John Innes Centre, Norwich, United Kingdom |
contact email | mattjstn@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038964 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038964
- Label: PRIDE project
- Name: Comparative phyloproteomics identifies conserved plasmodesmal proteins