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PXD038848

PXD038848 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn apicomplexan bromodomain, TgBDP1 associates with diverse epigenetic factors to regulate essential transcriptional processes in Toxoplasma gondii.
DescriptionThe protozoan pathogen Toxoplasma gondii relies on tight regulation of gene expression to invade and establish infection in its host. The divergent gene regulatory mechanisms of Toxoplasma and related apicomplexan pathogens rely heavily on regulators of chromatin structure and histone modifications. The important contribution of histone acetylation for Toxoplasma in both acute and chronic infection has been demonstrated, where histone acetylation increases at active gene loci. However, the direct consequences of specific histone acetylation marks and the signaling pathway that influences transcriptional regulation in response to the modification is unclear. As a reader of lysine acetylation, the bromodomain serves as a mediator between the acetylated histone and transcriptional regulators. Here we show that the bromodomain protein TgBDP1 which is conserved amongst Apicomplexa and within the Alveolata superphylum, is essential for Toxoplasma asexual proliferation. Using CUT&TAG we demonstrate that TgBDP1 is recruited to transcriptional start sites of a large proportion of parasite genes. Transcriptional profiling during TgBDP1 knockdown revealed that loss of TgBDP1 leads to major dysregulation of gene expression, implying multiple roles for TgBDP1 in both gene activation and repression. This is supported by interactome analysis of TgBDP1 demonstrating that TgBDP1 forms a core complex with two other bromodomain proteins and an ApiAP2 factor. This core complex appears to interact with other epigenetic factors such as nucleosome remodelling complexes. We conclude that TgBDP1 interacts with diverse epigenetic regulators to exert opposing influences on gene expression in the Toxoplasma tachyzoite.
HostingRepositoryPRIDE
AnnounceDate2023-06-01
AnnouncementXMLSubmission_2023-05-31_18:02:50.752.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSethMcNutt
SpeciesList scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-14 22:05:52ID requested
12023-05-31 18:02:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Toxoplasma, Gondii, Transcriptional, Bromodomain, Apicomplexan, Histone, Epigenetic, TgBDP1, Acetylation, LC-MS/MS
Contact List
FeixiaChu
contact affiliationDepartment of Molecular, Cellular, & Biomedical Sciences, Chu Lab, University of New Hampshire, United States
contact emailfeixia.chu@unh.edu
lab head
SethMcNutt
contact affiliationUniversity of New Hampshire
contact emailseth.mcnutt@unh.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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