PXD038779

PXD038779 is an original dataset announced via ProteomeXchange.

Title	Spastin is an essential regulator of male meiosis, acrosome formation, manchette structure and nuclear integrity
Description	The development and function of male gametes is critically dependent on a dynamic microtubule network, yet how this is regulated remains poorly understood. We have recently shown that microtubule severing, via the action of the meiotic AAA ATPase protein clade, plays a critical role in this process. Here, we sought to elucidate the roles of spastin, an as yet unexplored member of this clade in spermatogenesis. Using a SpastKO/KO mouse model, we reveal that spastin loss resulted in a complete loss of functional germ cells. Spastin plays a critical role in the assembly and function of the male meiotic spindle, and in its absence, apoptosis is significantly increased. Consistent with meiotic failure, round spermatid nuclei were enlarged, indicating aneuploidy, but were still able to enter spermiogenesis. During spermiogenesis, we observed extreme abnormalities in manchette structure, acrosome biogenesis, and commonly, a loss of nuclear integrity. This work defines a novel and essential role for spastin in regulating microtubule dynamics during spermatogenesis and is of potential relevance to patients carrying Spastin variants and to the medically assisted reproductive technology industry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:05.608.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Dunleavy
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 22:55:42	ID requested
1	2023-03-22 15:55:00	announced
⏵ 2	2023-11-14 09:00:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mouse, SPAST, testes, LC-MS/MS

Jessica Dunleavy
contact affiliation	School of BioSciences and Bio21 Institute, Faculty of Science, The University of Melbourne, VIC, 3010, Australia
contact email	jessica.dunleavy@unimelb.edu.au
lab head
Jessica Dunleavy
contact affiliation	The University of Melbourne
contact email	jessica.dunleavy@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038779

PRIDE project URI

• PRIDE
  1. PXD038779
     1. Label: PRIDE project
     2. Name: Spastin is an essential regulator of male meiosis, acrosome formation, manchette structure and nuclear integrity