PXD038631 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET |
Description | Gas Vesicles (GVs) are gas-filled protein nanostructures employed by several species of bacteria and archaea as flotation devices to enable access to optimal light and nutrients. The unique physical properties of GVs have led to their use as genetically-encodable contrast agents for ultrasound and MRI. Currently, however, the structure and assembly mechanism of GVs remain unknown. Here we employ cryo-electron tomography to reveal how the GV shell is formed by a helical filament of highly-conserved GvpA subunits. This filament changes polarity at the center of the GV cylinder, a site that may act as an elongation center. High-resolution subtomogram averaging reveals a corrugated pattern of the shell arising from polymerization of GvpA into a β-sheet. The accessory protein GvpC forms a helical cage around the GvpA shell, providing reinforcement. Together, our results help explain the remarkable mechanical properties of GVs and their ability to adopt different diameters and shapes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_11:11:39.395.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ting-Yu Wang |
SpeciesList | scientific name: Dolichospermum flos-aquae 04-10; NCBI TaxID: 749200; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-07 06:47:26 | ID requested | |
⏵ 1 | 2024-10-07 11:11:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cryo-ET.,Gas Vesicles |
Contact List
Grant J. Jensen |
contact affiliation | Division of Biology and Biological Engineering, California Institute of Technology,USA |
contact email | grant_jensen@byu.edu |
lab head | |
Ting-Yu Wang |
contact affiliation | Caltech |
contact email | tywang@caltech.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038631
- Label: PRIDE project
- Name: Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET