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PXD038631

PXD038631 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructure of Anabaena flos-aquae gas vesicles revealed by cryo-ET
DescriptionGas Vesicles (GVs) are gas-filled protein nanostructures employed by several species of bacteria and archaea as flotation devices to enable access to optimal light and nutrients. The unique physical properties of GVs have led to their use as genetically-encodable contrast agents for ultrasound and MRI. Currently, however, the structure and assembly mechanism of GVs remain unknown. Here we employ cryo-electron tomography to reveal how the GV shell is formed by a helical filament of highly-conserved GvpA subunits. This filament changes polarity at the center of the GV cylinder, a site that may act as an elongation center. High-resolution subtomogram averaging reveals a corrugated pattern of the shell arising from polymerization of GvpA into a β-sheet. The accessory protein GvpC forms a helical cage around the GvpA shell, providing reinforcement. Together, our results help explain the remarkable mechanical properties of GVs and their ability to adopt different diameters and shapes.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_11:11:39.395.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTing-Yu Wang
SpeciesList scientific name: Dolichospermum flos-aquae 04-10; NCBI TaxID: 749200;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 06:47:26ID requested
12024-10-07 11:11:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cryo-ET.,Gas Vesicles
Contact List
Grant J. Jensen
contact affiliationDivision of Biology and Biological Engineering, California Institute of Technology,USA
contact emailgrant_jensen@byu.edu
lab head
Ting-Yu Wang
contact affiliationCaltech
contact emailtywang@caltech.edu
dataset submitter
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Dataset FTP location
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