PXD038436 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics profiling reveals regulation of immune response to Salmonella enterica serovar Typhimurium infection in mice |
Description | Regulation of the immune response to Salmonella enterica serovar Typhimurium (S. Typhimurium) infection is a complex process, influenced by the interaction between genetic and environmental factors. Different inbred strains of mice exhibit distinct levels of resistance to S. Typhimurium infection, ranging from susceptible (e.g., C57BL/6J) to resistant (e.g., DBA/2J) strains. However, the underlying molecular mechanisms contributing to the host response remain elusive. In this study, we present a comprehensive proteomics profiling of spleen tissues from C57BL/6J and DBA/2J strains with different doses of S. Typhimurium infection by tandem tag mass coupled with two-dimensional liquid chromatography-tandem mass spectrometry (TMT-LC/LC-MS/MS). We identified and quantified 3,986 proteins, resulting in 475 differentially expressed proteins (DEPs) between C57BL/6J and DBA/2J strains. Functional enrichment analysis unveiled that the mechanism of innate immune responses to S. Typhimurium infection could be associated with several signaling pathways, including the interferon signaling pathway. We experimentally validated the roles of interferon signaling pathway in innate immune response to S. Typhimurium infection using IFN-γ neutralization assay. We further illustrated the roles of macrophage cells and pro-inflammatory cytokines in the mechanisms underlying the resistance to S. Typhimurium using qRT-PCR. Taken together, our results provide new insights into the genetic regulation of the immune response to S. Typhimurium infection in mice and might provide potential protein targets for controlling the infection. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:58:50.542.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ling Li |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-27 00:08:19 | ID requested | |
1 | 2022-12-01 12:05:12 | announced | |
⏵ 2 | 2023-11-14 08:58:51 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proteome |
Proteomics |
Mass Spectrometry |
Immune Response |
Genetic Regulation |
Salmonella |
S. Typhimurium |
Mouse |
Contact List
Xusheng Wang |
contact affiliation | Department of Biology, University of North Dakota, Grand Forks, ND 58202, USA |
contact email | xusheng.wang@UND.edu |
lab head | |
Ling Li |
contact affiliation | University of North Dakota |
contact email | ling.li.1@und.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038436
- Label: PRIDE project
- Name: Proteomics profiling reveals regulation of immune response to Salmonella enterica serovar Typhimurium infection in mice