PXD038264 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The role of endosomal protein DENND10/FAM45A in extracellular vesicle release and cancer cell migration |
Description | Cell migration is key to tumor metastasis, the most challenging clinical problem in cancer treatment. Here we are investigating the role of DENND10/FAM45A, a recently described endosomal protein, in cancer cell migration via modulating the release of extracellular vesicles (EVs). Bioinformatics data mining showed that high DENND10 expression is significantly associated with poor prognosis in multiple cancer types. DENND10 knockout (DENND10-KO) resulted in defective cell spreading and migration, with impaired formation of stress fibers and focal adhesions. EV secretion was reduced in DENND10-KO cells. Importantly, wild-type conditioned medium or EVs restored the migratory ability and cytoskeletal organization of DENND10-KO cells. Quantitative mass spectrometry revealed that DENND10 depletion led to distinct EV compositional profiles with remodeled constituents of adhesion molecules and extracellular matrix. Together, our study unveiled DENND10 as an intrinsic coordinator between EV release and cell migration, which could be exploited for designing new anti-cancer strategies. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:44:55.578.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yanling Zhang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-22 02:44:43 | ID requested | |
1 | 2024-06-14 00:58:09 | announced | |
⏵ 2 | 2024-10-22 06:44:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: extracellular vesicle,Mouse, DENND10, mammary cancer, 4T1 |
Contact List
Yanling Zhang |
contact affiliation | Soochow University |
contact email | yanlzhan@suda.edu.cn |
lab head | |
Yanling Zhang |
contact affiliation | Soochow University |
contact email | yanlzhan@suda.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038264
- Label: PRIDE project
- Name: The role of endosomal protein DENND10/FAM45A in extracellular vesicle release and cancer cell migration